OVERVIEW

Shimadzu Spectrophotometer UV-1280: Designed by the leaders in UV-Visible Spectroscopy for molecular absorption quantitative analysis, the UV-1280 Multipurpose UV-Visible Spectrophotometer offers wavelength scanning from 190-1100nm. This lower-cost, high-quality instrument is ideal for applications ranging from routine environmental and food quality testing to life science analyses.

UV-1280 + DPU-S445 screen copy printer (option)

Prints hard copies of screens, including numerical data. It also allows printing of the items displayed on the screen, such as spectra and calibration curves.

UV-1280 + Commercial printer (option)

Printing is possible to printers that support ESC/P-9, ESC/P-24, ESC/P Raster, and PCL control codes.Instrument

Maintenance and Inspections

Instrument validation and maintenance/inspection functions have been enhanced. The usage times of the deuterium (D2) lamp and the halogen (WI) lamp can be recorded and displayed, which enables users to ascertain the expected replacement period of the lamps when performing periodic inspections. The checks for eight JIS items can be performed automatically or semi-automatically. Linking with a 6-series multi-cell (optional) is possible, making sample replacement unnecessary. Inspections can be performed efficiently.

Instrument Maintenance and Inspections

Instrument validation and maintenance/inspection functions have been enhanced. The usage times of the deuterium (D2) lamp and the halogen (WI) lamp can be recorded and displayed, which enables users to ascertain the expected replacement period of the lamps when performing periodic inspections.

A Variety of High-Level Measurement Modes

Photometric Mode

Measures the absorbance or transmittance at a single wavelength or multiple (up to eight) wavelengths. The instrument is also capable of simple quantitation using the K-factor method. For a multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths.

Spectrum Mode

A sample spectrum is recorded using wavelength scanning. Repeat scans let you follow sample changes over time. Zoom in on the finished spectrum for a better view; subsequently, use the peak/valley pick function to select maxima and minima and perform a wide variety of data processing functions.

Quantitation Mode

Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns. Allows various combinations of wavelength number (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order).

Kinetics Mode

Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method automatically calculates the amount of change per minute, and then calculates an activity value from a specified coefficient. The rate measurement method, which determines whether the absorbance is changing linearly, can also be selected. In addition, add the CPS-100 thermoelectrically temperature-controlled cell positioner for measurement of multiple samples in succession.

Time Scan Mode

Measures the change in absorbance, transmittance or energy as a function of time. Add the CPS-100 thermoelectrically temperature-controlled cell positioner for simultaneous measurement of multiple samples under constant-temperature conditions.

Multi-Component Quantitation Mode

Quantitates up to eight components mixed in a single sample. The calibration equation is determined using pure or mixed components with known values.

Biomethod Mode

Determine DNA and protein concentrations with the following quantitation methods using the bioscience/life science program included as standard.
DNA/Protein Quantitation
・Quantitates DNA or protein using the absorbance at 260/230 nm or
260/280 nm.
Protein Quantitation
• Lowry method
• BCA method (method using bicinchoninic acid)
• Biuret method
• CBB method (method using Coomassie Brilliant Blue G-250)
• UV absorption method (direct measurement at 280 nm)

Using USB flash drives makes it easy to transport analysis data and to store large amounts of data in a PC.
USB flash drives can be connected directly to the UV-1280.
Data for spectra and time-course curves can be displayed and saved with commercial spreadsheet software.
With the UV-1280, the curve-related data (spectra and time-course curves) can be converted to, and saved in,
CSV format. Transferring this data with USB flash drives allows it to be read directly at a PC using commercial spreadsheet software. UV-1280 + USB flash drive + PC

Shimadzu Spectrophotometer UV-1280