OVERVIEW

Shimadzu Spectrophotometer UV-1900i: Easy-to-Use Interface Grasp the Current Status and Operating Procedures at a Glance

The UV-1900i on-screen user interface includes large, easy-to-see icons deployed on a black background, so the instrument settings are evident at a glance. In addition, the large, easy-to-see icons improve intuitive understanding, which enables users to quickly become familiar with the operations. Furthermore, the user interface is designed to minimize transitions between windows, so users do not get confused during the operations.

Ergonomic Hardware Design

The control panel is ergonomically designed and positioned at the optimum viewing angle for the user. Users can operate easily with any posture. Since the panel is pressure-sensitive, the device can be operated with gloved fingers or a stylus pen. The stylus pen can be stored right next to the control panel.

Navigation Tabs Improve Usability

In quantitation mode on the UV-1900i, the stages of the entire measurement process and the current status are always shown on the display. As a result, users know immediately what to do in the next step.

Low Stray Light

Stray light is at 0.5 % max. (198 nm), making accurate measurements are possible up to the vicinity of 2 Abs even in the ultraviolet region. In addition, high-concentration samples can be quantified accurately. The figure on the right is a calibration curve for acetic acid, created with absorbance at 200 nm.
The correlation coefficient is 0.9997 and correct measured values are obtained even in the vicinity of 2 Abs. Linearity will be lost in the high absorbance region due to the stray light.

Ultra-Fast Scan

Spectra can be acquired as fast as 29,000 nm/min. Ultra-fast scan is effective in tracking chemical reactions in a short time. In addition to the absorbance change at specified wavelengths, spectra can also be acquired in a short time with the UV-1900i. Therefore, more detailed behavior can be investigated by observing spectra with the UV-1900i. The figures below show the analysis of the particle agglomeration process when salts are added to silver nanoparticles. Measurements of the 300 to 700 nm region were performed in ultra-fast scan mode. In addition to the decrease of absorbance at 400 nm and the increase of absorbance at 480 nm, the temporal changes of spectra can also be observed.

High Reproducibility and Repeatability Accuracy

The photometric repeatability accuracy is 0.0002 Abs max. (0.5 Abs and 1.0 Abs). With this high photometric repeatability accuracy, variance in the measurement results is suppressed, enabling more accurate quantitation and the detection of low-concentration samples.

Network Connectivity Function

The UV-1900i provides network access via wireless connectivity.

Data can now be transferred to a PC via a network.
Thanks to wireless printing, multiple UV units can print from a single printer.
(A router and other network equipment must be installed to use a network.)

Sleep Mode and Wakeup Function

Analysis can start the instant the user arrives at the laboratory in the morning. The instrument requires no time to warm up.

Bar-Code Reader and Keyboard Entry Function

Sample names and numerical values can be entered by a bar-code reader or from the keyboard. This saves time when entering sample names for a multiple sample analysis, and prevents sample misidentification and other human errors.*3

Shimadzu Spectrophotometer UV-1900i A Diversity of Measurement Modes 

Photometric

Measures the photometric value at a single wavelength or multiple (up to eight) wavelengths.

Spectrum

Measures a sample spectrum using wavelength scanning.

Quantitation

Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns.

Kinetics

Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method or the rate measurement method can be selected.

Time Course

Measures changes over time in photometric values at a specified wavelength.

Biomethod

Quantifies DNA or protein concentrations.

Shimadzu Spectrophotometer UV-1900i Applications 

Foods

This is an example of the analysis of food dyes. By using ultra-fast scan mode, the time needed for routine spectral checks can be shortened. The 350 nm to 900 nm region can be measured at 1 nm intervals in approx. 4 seconds.

Pharmaceuticals and Life Sciences

This is an example of the analysis of DNA. Trace quantities (on the order of a few μL) can be measured by combining the instrument with Nano Stick and TrayCell®. Using TrayCell, a calibration curve for 4 μL of DNA was
obtained in the range between 25 ng/μL and 500 ng/μL.

Chemistry

This is an example of the analysis of colored cellophane tape. Materials can be confirmed quantitatively by using LabSolutions UV-Vis and color measurement software.